Resolution of microsomal membranes into fractions differing in polypeptide composition |
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| Authors: | Mustak Ali Kaderbhai Robert B. Freedman |
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| Affiliation: | Biological Laboratory, University of Kent, Canterbury, Kent CT2 7NJ U.K. |
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| Abstract: | Microsomes from rat liver, prepared by gel filtration, were subjected to centrifugation in a continuous sucrose density gradient containing a low concentration of deoxycholate. The membranes were subfractionated into five bands differing in appearance and equilibrium density. Each band, when analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis, displayed a characteristic population of membrane proteins. |
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| Keywords: | Membrane fractionation Membrane protein Microsome Polypeptide composition SDS sodium dodecyl sulphate |
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