Contribution of whole cell and cytoplasmic polypeptides to apparent red cell membrane alterations |
| |
Authors: | Nancy Sauberman LMichael Snyder |
| |
Institution: | Hematology Division, St. Vincent Hospital, 25 Winthrop St., Worcester, MA 01604 U.S.A. |
| |
Abstract: | We have compared densitometric tracings of whole cell, cytoplasmic and membrane polypeptide electrophoretic patterns in an attempt to distinguish atypical partitioning from intrinsic membrane polypeptide changes occurring as a result of reticulocyte enrichment, metabolic depletion, treatment and hereditary xerocytosis. We report that membrane alterations seen in a reticulocyte-enriched population of normal cells are present in the whole cells prior to membrane isolation. Some of the membrane alterations in metabolically depleted cells and all of those in cells are traced to modifications in the partitioning of polypeptides between membranes and supernatant (cytoplasm) at hemolysis.The power of this approach in resolving the sources of apparent red cell membrane protein alterations is demonstrated in studies with hereditary xerocytes. Suggested altered partitioning of these cells described earlier (Sauberman, N., Fortier, N.L., Fairbanks, G., O'Connor, R.J. and Snyder, L.M. (1979) Biochim. Biophys. Acta 556, 292–313) is further documented and found to be unrelated to the younger cell population or slight metabolic depletion that occurs during the washing of xerocytes prior to hemolysis. |
| |
Keywords: | Aging Membrane protein Metabolic depletion Xerocytosis N-Ethylmaleimide (Red cell) |
本文献已被 ScienceDirect 等数据库收录! |
|