Application of 31P-NMR saturation transfer techniques to investigate phospholipid motion and organization in model and biological membranes |
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| Authors: | B De Kruijff GA Morris PR Cullis |
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| Institution: | 1. Department of Biochemistry, University of British Columbia, Vancouver, B.C. Canada;2. Department of Chemistry, University of British Columbia, Vancouver, B.C. Canada |
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| Abstract: | The potential of 31P-NMR saturation transfer experiments for determining motional characteristics (in the millisecond to second time scale) of phospholipids in model and biological membranes is demonstrated. A technique to separate membrane phospholipid 31P-NMR signals from those of small water-soluble phosphates in intact cells in liver tissue is also illustrated. |
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| Keywords: | Saturation transfer Phospholipid organization Motional characteristics Lateral diffusion |
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