Perturbations to lipid bilayers by spectroscopic probes as determined by dielectric measurements |
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Authors: | RG Ashcroft KR Thulborn JR Smith HGL Coster WH Sawyer |
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Institution: | 1. School of Physics, University of New South Wales, Sydney, N.S.W. Australia;2. Russel Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria Australia |
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Abstract: | Dielectric measurements on lecithin/cholesterol bimolecular lipid membranes have indicated that the series of extrinsic fluorescent probe molecules, the fatty acids, cause significant perturbation to the bilayer structure at concentrations equivalent to those used in fluorescence experiments (0.1 mol%). Perturbations were observed in the capacitance and conductance of the electrically distinct substructural regions of the bilayer that were consistent with the putative location of the probe molecules. Inclusion of stearic acid decreased the thickness of the hydrocarbon region of the membrane, presumably by expanding the average surface area per unit membrane mass, and also significantly disrupted the surface regions. The attachment of the anthroyloxy moiety to position 2 of a fatty acid accentuated both these effects. Attachment at position 12 had the reverse effect by increasing the volume of the hydrocarbon region without further disturbance of the surface organisation. The 9-positioned probe had an intermediate effect. The degree of perturbation by the 2-positioned probe was dependent on the probe concentration within the range (probe:lipid) 1:1000 to 1:10 000. The technique therefore detects perturbation of structure at probe levels which are lower than those commonly used in fluorescence-labelling experiments. |
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Keywords: | Perturbation Dielectric dispersion Fluorescent probe n-(9-Anthroyloxy) fatty acid Impedance Membrane structure (Planar bilayer) |
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