Solubilization of 20S acetylcholinesterase from chick retina |
| |
Authors: | Ana Barat Elena Escudero Javier Gómez-Barriocanal Galo Ramirez |
| |
Institution: | Centro de Biología Molecular, CSIC-UAM, Canto Blanco, Madrid-34, Spain. |
| |
Abstract: | A 20S form of acetylcholinesterase has been solubilized from young chick retinas by means of a buffered salt-detergent solution containing EDTA. The release of this fast-sedimenting form of the enzyme is selectively blocked by the presence of even small amounts of Ca++ in the homogenization medium. The collagen-tailed nature of this molecular species of acetylcholinesterase has been ascertained by collagenase digestion. This finding suggests that the avian central nervous system contains asymmetric, collagen-tailed quaternary structural forms of acetylcholinesterase as is the case in skeletal muscle and cholinergic ganglia. |
| |
Keywords: | To whom correspondence should be addressed |
本文献已被 ScienceDirect 等数据库收录! |