Light-enhanced cross-linking of rhodopsin in rod outer segment membranes as detected by chemical probes |
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| Authors: | Arthur Shaw,Richard Crain,Guido V. Marinetti,David O&#x Brien,Patricia N. Tyminski |
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| Affiliation: | 1. Department of Biochemistry, University of Rochester Medical Center, Rochester, NY 14642 U.S.A.;2. Research Laboratories, Eastman Kodak Company, Rochester, NY U.S.A. |
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| Abstract: | Bovine rod outer segment membranes were treated with cross-linking reagents before and after light exposure. Bleached membranes showed enhanced cross-linking with difluorodinitrobenzene or methyl acetimidate compared to dark-adapted membranes. The light-induced enhancement of cross-linking may be due to increased association of rhodopsin monomers in the light and/or due to increased reactivity of amino and sulfhydryl groups of bleached rhodopsin. In some instances, the band ascribed to the rhodopsin monomer in gel electrophoresis appears as a partially resolved doublet. Treatment of bleached rod outer segment membranes with methyl acetimidate improved the resolution of the doublet into two closely migrating bands. |
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| Keywords: | Cross-linking Rhodopsin Light enhancement Chemical probe (Rod outer segment) |
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