Thin-layer chromatography with agarose gels: A quick,simple method for evaluating liposome size |
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| Authors: | AJBM van Renswoude R Blumenthal JN Weinstein |
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| Institution: | 1. Laboratory of Physiological Chemistry, State University of Groningen, Medical School Bloemsingel 10, 9712 KZ Groningen The Netherlands;2. Laboratory of Theoretical Biology, National Cancer Institute, NIH, Bethesda, MD 20205 U.S.A. |
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| Abstract: | Thin-layer gels can be made with agarose and used to assess within a few minutes the efficiency with which multilamellar vesicles are converted to small unilamellar ones by sonication. A fluorescent lipid marker or vesicle-encapsulated solute permits continuous monitoring of the chromatography. Advantages over agarose gel column chromatography include speed of analysis, small sample size, the possibility of running multiple samples simultaneously, and direct accessibility to fluorescence microscopy. This approach should also be useful in the study of liposome-lipoprotein interactions and in affinity chromatography of liposomes. |
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| Keywords: | Liposome size Agarose gel (TLC) |
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