Vanadate inhibits 2,3-bisphosphoglycerate dependent phosphoglycerate mutases but does not affect the 2,3-bisphosphoglycerate independent phosphoglycerate mutases |
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| Authors: | José Carreras Ramón Bartrons Santiago Grisolía |
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| Institution: | Departamento de Bioquimíca. Facultad de Medicina. Universidad de Barcelon Casanova 143. Barcelona-36 Spain;Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia Amadeo de Saboya 4. Valencia-10 Spain |
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| Abstract: | There are two types of phosphoglycerate mutases. The 2,3-bisphosphoglycerate dependent phosphoglycerate mutases are inhibited by vanadate. In contrast, the 2,3-bisphosphoglycerate independent mutases are not affected. The effect of vanadate varies with pH, and can be reversed by dilution, EDTA and norepinephrine. The differential effect of vanadate on the two types of phosphoglycerate mutases supplies a novel way to easily differentiate both types of enzymes. In addition, it may contribute to the clarification of the mechanism of action of the 2,3-bisphosphoglycerate independent phosphoglycerate mutases. |
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