Faculty of Pharmaceutical Sciences, Chiba University, Yayoi-cho, Chiba 260, Japan
Abstract:
It is shown that the stimulation of eukaryotic polypeptide synthesis by spermidine is due to the stimulation at the level of initiation by following reasons. The incorporation of formylmethionine into polypeptides was stimulated by spermidine at the same degree to the incorporation of leucine into polypeptides. Fluorography of the polypeptides formed showed that the number of chains of individual protein synthesized was larger when spermidine was added. The formation of the complex of Met-tRNAf, globin mRNA and 40-S ribosomal subunits was stimulated by spermidine.