Faithful and efficient translation of viral and cellular eukaryotic mRNAs in a cell-free S-27 extract of <span class="math"><math><mtext>Saccharomyces</mtext><mtext>cerevisiae</mtext></math></span>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Faithful and efficient translation of viral and cellular eukaryotic mRNAs in a cell-free S-27 extract of $Saccharomyces cerevisiae$

Authors:	Elżbieta Szczęsna Witold Filipowicz

Institution:	Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 36 Rakowiecka str., 02-532 Warsaw, Poland

Abstract:	The preparation of yeast spheroplast 27,000 × g supernatant /S-27/ which initiates translation of endogenous and exogenous mRNAs is described. The activity of this protein synthesis system is comparable to that of the most efficient cell-free extracts currently in use. The yeast S-27 system is able to carry out faithful translation of distinct eukaryotic mRNAs into proteins as large as 180,000 daltons.

Keywords:	S-27 27 000 × g supernatant SDS sodium dodecyl sulphate DTT dithiothreitol 7-methylguanosine-5′-monophosphate TMV tobacco mosaic virus PVX potato virus X AMV alfalfa mosaic virus TYMV turnip yellow mosaic virus
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