Effects of temperature on L-leucine transport in toadfish liver in vivo |
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Authors: | Roger Persell Audrey EV Haschemeyer |
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Institution: | Department of Biological Sciences, Hunter College of the City University of New York, Box 741, New York, NY 10021 U.S.A. |
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Abstract: | The effect of body temperature in the 4–30°C range on L-leucine uptake by toadfish liver in vivo was examined by means of a single-injection pulse technique. The ratio of 14C]leucine to 3H]mannitol or 3H]inulin in blood leaving the liver was measured as a function of time after hepatic portal vein injection. Recoveries of the two isotopes in liver and 14C]leucine incorporation into protein were determined.The Q10 value for influx was 3.8, that for efflux 2.8. At all temperatures, the leucine influx was 8–10-times higher than its incorporation into protein. The directly energy-linked reactions appear to be the main site of increased temperature sensitivity at low temperatures. |
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Keywords: | Amino acid transport Temperature effect Poikilothermic organism (Liver) |
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