Demethylation of O6-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichiacoli |
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Authors: | RS Foote S Mitra BC Pal |
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Institution: | 1. University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tennessee 37830 USA;2. the Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830 USA |
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Abstract: | 6-Methyl8-3H]deoxyguanosine in a synthetic DNA polymer, poly(dC, dG, m6dG), is demethylated by cell-free extracts of adapted by exposure to -methyl-′-nitro--nitrosoguanidine, as shown by the appearance of 3H-labeled deoxyguanosine in hydrolysates of the recovered DNA. The demethylating activity could not be detected in extracts of nonadapted . These results provide direct evidence that a previously described inducible repair activity in acts by demethylating 6-methylguanine at the DNA level. |
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