Antibacterial functionalization of wool fabric via immobilizing lysozymes |
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Authors: | Qiang Wang Xuerong Fan Yingjun Hu Jiugang Yuan Li Cui Ping Wang |
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Institution: | (1) Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China |
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Abstract: | Greater attention has been given to enzymatic processes of textiles as effective alternatives to conventional chemical treatments
because of the non-toxic and eco-friendly characteristics of enzymes as well as the increasingly important requirement for
reducing pollution in textile production. A new functionalization method for wool fabrics based on immobilization of lysozymes
was investigated in this paper. Wool fabric was first activated with glutaraldehyde, and then employed to covalently immobilize
lysozymes. Main immobilization parameters were optimized in terms of the activity of immobilized enzyme. A high activity of
the immobilized enzyme was obtained when the fabric was activated at 25 °C for 6 h in a bath containing with 0.2% of glutaraldehyde
followed by the immobilization at 4 °C and pH 7.0 for 6 h with 5 g l−1 lysozyme. The scanning electron microscopy and staining tests based on modified Coomassie protein assay (Bradford method)
revealed that the lysozyme was fixed covalently on the wool fabric. Wool fabrics immobilizing lysozymes presented a higher
ratio of bacteriostasis to Staphylococcus aureus. The durability of antibacterial wool was assessed and the lysozyme immobilized on wool fabric retained ca. 43% of its activity
after five cycles of use when taking the activity of the immobilized lysozyme before using as reference. |
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Keywords: | Lysozyme Immobilization Antibacterial functionalization Wool |
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