Permeabilization of<Emphasis Type="Italic">Ochrobactrum anthropi</Emphasis> SY509 cells with organic solvents for whole cell biocatalyst |
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| Authors: | Kyung?Oh?Choi Seung?Hoon?Song Email author" target="_blank">Young?Je?YooEmail author |
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| Institution: | (1) School of Chemical Engineering, Seoul National University, 151-742 Seoul, Korea;(2) Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, 151-742 Seoul, Korea |
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| Abstract: | Permeabilization is known to overcome cell membrane barriers of whole cell biocatalysts. The use of organic solvents is advantageous
in terms of cost, simplicity, and efficiency. In this study,Ochrobactrum anthropi SY509 was permeabilized with various organic solvents. Treatment with organic solvents resulted in lower permeability barriers
due to falling out lipids of the cell membrane. Therefore, permeabilized cells showed higher enzyme activity with no cell
viability. Among various organic solvents, 0.5% (v/v) chloroform was selected as the most efficient permeabilizing reagent.
Changes in the cell membrane structure were observed and the residual amounts of phospholipids of the cell membrane were measured
to investigate the mechanism of the improved permeability. |
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| Keywords: | permeabilization permeability barrier organic solvent Ochrobactrum anthropi phospholipids |
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