Cyclic-voltammetric determination of cysteine in rat organs after intravenous injection |
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Authors: | J Pradáč J Pradáčová J Koryta |
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Institution: | 1 Institute of Biophysics, Charles University, Salmovská 3, Prague 2, Czechoslovakia;1. J. Heyrovský Institute of Polarography, Czechoslovak Academy of Sciences, Opletalova 25, Prague 1, Czechoslovakia |
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Abstract: | A method of cyclic voltammetry was used for the determination of cysteine in rat organs in vivo after its intravenous injection. The cyclic polarization enabled a periodic reactivation of the platinum test electrode surface so that reproducible results could be obtained. The peaks of the I-E curve which were due to oxidation of cysteine depended for reproducibility on the concentration of cysteine injected. We attempted to use the method described for the assessment of kidney tissue blood supply before transplantation. |
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Keywords: | NHE normal hydrogen electrode |
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