Adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate: A simultaneous protein binding assay |
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Authors: | Ferid Murad Alfred G Gilman |
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Institution: | 1. Department of Medicine and Pharmacology, University of Virginia, School of Medicine, Charlottesville, Va. 22903, U.S.A.;2. Laboratory of Biochemical Genetics, National Heart and Lung Institute, Bethesda, Md. 20014, U.S.A. |
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Abstract: | The adenosine 3′,5′-monophosphate and guanosine 3′,5′-monophosphate contents of microliter quantities of urine can be determined simultaneously by combining individual protein binding assays for the two nucleotides. 32P-labeled adenosine 3′,5′-monophosphate is bound to a protein from bovine skeletal muscle, while a lobster muscle protein preparation is utilized for binding of 3H-labeled guanosine 3′,5′-monophosphate. |
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Keywords: | cyclic AMP adenosine 3&prime 5&prime -monophosphate cyclic GMP guanosine 3&prime 5&prime -mophosphate |
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