A mathematical model of the course of the DNA synthesis in mammalian cells after ultraviolet irradiation and its use in the determination of the length of the replicon

The chromosome of mammalian cells is divided into several shorter segments — replicons which replicate separately. When the cells are irradiated with ultraviolet radiation the pyrimidine flimers preventing the DNA-polymerase in its normal function and creating obstacles for a timely completing of the implication are formed. Using these facts as a basis we have derived a mathematical model of the course of the DNA synthesis in mammalian cells after ultraviolet irradiation, it has been proved that for the determination of the average length of units of replication in chromosomes of these cells the measurement of the intensity of post-radiation DNA synthesis in an asynchronous culture of mammalian cells can be used. Directions for the determination of this length using results of the measurement of the post-radiation synthesis are given, too. The whole method has already beer, tested on mammalian L-cells.