Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior |
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| Authors: | Sindarovska Y R Sheludko Y V Gerasymenko I M Bannikova M A Kuchuk N V |
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| Institution: | Institute of Cell Biology and Genetic Engineering, Kyiv, Ukraine. |
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| Abstract: | Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium-mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification scheme has been developed and included ammonium sulfate precipitation and Q-sepharose anion-exchange chromatography. It results in obtaining of a fraction with about 85% GFP homogeneity and the protein yield of about 75%. |
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