Relationship between the rate of erythrocyte hexose monophosphate pathway and the glucose 6-phosphate concentration |
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Authors: | M Magnani V Stocchi A Fazi M Dachà G Fornaini |
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Affiliation: | Istituto di Chimica Biologica, Università degli Studi, Urbino, Italy |
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Abstract: | Erythrocytes of individuals with increased (+ 50%) or reduced (-35%) hexokinase activity contain respectively 70 and 17 nmole/ml RBC of glucose-6-phosphate (normal concentration 30 +/- 5nmole/ml RBC) and show comparable rates of the HMP (60 +/- 5nmole/hr/ml RBC). Similarly, in RBC of different ages, obtained by density gradient ultracentrifugation, the glucose-6-phosphate concentration range from 57 (young cells) to 18 (old cells) nmole/ml RBC but the rate at which glucose is utilized in the HMP is unchanged. These data exclude a regulatory role of glucose 6-phosphate in the HMP even if its concentration is under that required for maximal G6PD activity. |
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Keywords: | RBC Red blood cells HMP hexose monophosphate pathway G6PD glucose 6-phosphate dehydrogenase Hb hemoglobin |
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