Evaluation of environmental conditions for production of bacteriocin-like substance by <Emphasis Type="Italic">Bacillus</Emphasis> sp. strain P34 |
| |
Authors: | Amanda S Motta Adriano Brandelli |
| |
Institution: | 1.Departamento de Ciência dos Alimentos, Laboratório de Bioquímica e Microbiologia Aplicada,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil;2.ICTA-UFRGS,Porto Alegre,Brazil |
| |
Abstract: | The influence of temperature, pH and media on bacteriocin production by Bacillus sp. P34 was investigated. The effect of temperature and initial pH was evaluated by factorial design and response surface
methodology (RSM). Statistical analysis of results showed that, in the range studied, the two variables have a significant
effect on bacteriocin production. Response-surface data showed maximum antimicrobial activity production at initial pH values
between 6.0 and 8.0 and temperatures between 25 and 37 °C. No relationship between bacterial growth and bacteriocin production
was observed. RSM proved to be a powerful tool in optimizing the production of antimicrobial activity by Bacillus sp. P34. When different media were tested, maximum bacteriocin production was observed in soybean protein-based medium, but
antimicrobial activity was not achieved by cultivation on fish meal, feather meal, whey and grape waste. |
| |
Keywords: | Antimicrobial activity Bacteriocin Byproducts Experimental design Listeria monocytogenes Statistical modeling |
本文献已被 SpringerLink 等数据库收录! |
|