| RNA干扰技术对肝癌细胞内源survivin基因表达的影响 |
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| 引用本文： | 闫歌,蒲丹,唐霓,高小玲,宋文鑫,卢年芳,吴刚,TONG-CHUAN HE,黄爱龙. RNA干扰技术对肝癌细胞内源survivin基因表达的影响[J]. 生物化学与生物物理进展, 2004, 31(9): 829-833 |
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| 作者姓名： | 闫歌 蒲丹 唐霓 高小玲 宋文鑫 卢年芳 吴刚 TONG-CHUAN HE 黄爱龙 |
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| 作者单位： | 1. 重庆医科大学病毒性肝炎研究所,重庆,400010
2. The University of Chicago Medical Center,Chicago,IL 60637,USA |
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| 基金项目： | 国家自然科学基金资助项目（30371599）和国家杰出青年基金资助（B类）（30228026）. |
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| 摘 要： | 应用RNA干扰技术（RNAi）研究针对凋亡抑制因子survivin的siRNA抑制肝癌细胞株内源survivin基因的表达.转染重组质粒pshRNA-survivin至肝癌细胞株SMMC-7721，通过免疫荧光、蛋白质印迹和半定量RT-PCR检测survivin蛋白表达及mRNA转录水平的变化.结果表明：构建的三种重组质粒pshRNA-survivin1/2/3均能明显抑制survivin基因的表达；应用免疫荧光检测survivin基因的表达，转染重组质粒pshRNA-survivin的实验组survivin荧光强度明显低于转染载体pTZU6＋1和pshRNA-GFP对照组；蛋白质印迹结果表明，重组质粒pshRNA-survivin明显抑制survivin蛋白的表达，抑制率为62%～78％，通过半定量RT-PCR检测到survivin基因mRNA转录明显减少，抑制率为57%～64％.由上述结果可以得出结论：重组质粒pshRNA-survivin可明显抑制SMMC-7721细胞内源survivin的表达和mRNA的转录，为survivin介导的肿瘤基因沉寂疗法提供实验基础.
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| 关 键 词： | RNA干扰，肝癌细胞株，survivin基因 |
| 收稿时间： | 2004-03-25 |
| 修稿时间： | 2004-04-06 |
RNA Interference-mediated Inhibition of Endogenous Survivin Expression in Hepatocarcinoma Cell |
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| YAN Ge,PU Dan,TANG Ni,GAO Xiao-Ling,SONG Wen-Xin,LU Nian-Fang,WU Gang,TONG-CHUAN HE and HUANG Ai-Long. RNA Interference-mediated Inhibition of Endogenous Survivin Expression in Hepatocarcinoma Cell[J]. Progress In Biochemistry and Biophysics, 2004, 31(9): 829-833 |
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| Authors: | YAN Ge PU Dan TANG Ni GAO Xiao-Ling SONG Wen-Xin LU Nian-Fang WU Gang TONG-CHUAN HE HUANG Ai-Long |
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| Affiliation: | Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China;Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China;Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China;Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China;Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China;Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China;Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China;The University of Chicago Medical Center, Chicago, IL 60637,USA;Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China |
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| Abstract: | |
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| Keywords: | RNA interference hepatocarcinoma cell survivin gene |
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