Competition studies withRhizobium trifolii in laboratory experiments |
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Authors: | T. J. McLoughlin L. K. Dunican |
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Affiliation: | (1) Agrigenetics Advanced Research Division, 5649 East Buckeye Road, 53716 Madison, Wisconsin, USA;(2) Department of Microbiology, University College, Galway, Ireland |
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Abstract: | Summary Competition studies were carried out in soil cores by comparing the nodule forming ability of antibiotic resistance marked strains, inoculated at three inoculum levels onTrifolium repens versus an effective indigenous Rhizobium population of 1.5×105/gm. It was seen that the indigenous marked isolate G1067 formed a high proportion of nodules sampled (>90%) at all three inoculum levels (105, 107 and 109 cells/seed) wherein the introduced foreign strain G1032 formed >50% of the nodules at the highest inoculum level.In a test tube experiment, competition for nodule sites was examined by inoculating mixtures of twoR. trifolii strains at different input levels on two cultivars ofTrifolium repens var. Huia and Titan. It was seen that G1032 was less competitive than G1006 and G1067 on cv. Huia but was more competitive on cv. Titan than the other two strains. |
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Keywords: | Competition studies Rhizobium trifolii Soil cores Trifolium repens |
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