Indirect organogenesis from leaf explants of Hemidesmus indicus (L.) R. Br.: an important medicinal plant |
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| Authors: | A. R. Pathak |
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| Affiliation: | Department of Botany, Faculty of Science, The M.S. University of Baroda, Vadodara, India |
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| Abstract: | Hemidesmus indicus (Asclepiadaceae) leaf explants were utilized for establishing culture in MS medium fortified with individual cytokinins, auxins, and their combinations. Optimum response (80%) was observed in N6-benzyladenine (BA, 20 μM) + indole-3-acetic acid (IAA, 1 μM) with 19.67 ± 0.81 shoots per explant. Roots were induced in ¼MS + indole-3-butyric acid (IBA, 20 μM). |
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| Keywords: | Hemidesmus indicus indirect organogenesis leaf explant Murashige and Skoog’s medium shoot cultures |
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