Micropropagation of Plumbago rosea Linn. |
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Authors: | K. Satheesh Kumar K. V. Bhavanandan |
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Affiliation: | (1) Department of Botany, University of Kerala, 695 581 Kariavattom, Kerala, India |
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Abstract: | Multiple shoot formation from the medicinal plant Plumbago rosea Linn. was induced on callus from stem segments on Murashige & Skoog media containing auxin and cytokinin. 2,4-D (2.5 mg l-1) and kinetin (1.5 mg l-1) added to the media gave best callus production, while BAP (2 mg l-1) plus NAA (1.0 mg l-1) induced shoot formation from that callus. Numerous shoots with roots could be produced by transferring shoots to media containing IBA (1.5 mg l-1). Regenerated plantlets were transferred to pots and 60% survived. |
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Keywords: | stem explants multiple shoots Plumbago rosea medicinal plants |
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