Characterization of two galactosidases extracted from wheat germ with a hydroalcoholic solvent. |
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| Authors: | M P Papet D Delay M Monsigny F Delmotte |
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| Affiliation: | Département de Biochimie des Glycoconjugués et des Lectines Endogènes, CNRS, Orléans, France. |
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| Abstract: | Alpha- and beta-D-galactosidases were characterized from a hydroalcoholic extract of wheat germ (Triticum vulgare). Kinetic constants (Vmax and KM) and the optimal pHs for the hydrolysis of p-nitrophenyl galactopyranosides by both enzymes were determined. These enzymes presented a high stability in hydroalcoholic medium and were inhibited by iodoacetamide and sodium p-hydroxy-mercuribenzoate. |
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