Study of molecular genetic diversity and evolutionary history of medicinally important endangered genus Chlorophytum using DNA barcodes |
| |
| Institution: | 1. Department of Biotechnology, Shivaji University, Kolhapur 416004, India;2. Department of Botany, Shivaji University, Kolhapur 416004, India;3. Department of Biochemistry, Shivaji University, Kolhapur 416004, India;1. Department of Pharmacology and Pharmacognosy, Government College of Pharmacy, Karad, India;2. Department of Pharmacology, Appasaheb Birnale College of Pharmacy, India;3. Department of Pharmacology, Rajarambapu College of Pharmacy, Kasegaon, India;1. School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Ministry of Education), Yantai University, Yantai 264005, China;2. School of Life Sciences, Yantai University, Yantai 264005, China;3. Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University, Yongin, Republic of Korea;1. Department of Obstetrics and Gynecology, Ege University, Bornova, Izmir, Turkey;2. Department of Physiology, Ege University, 35100 Bornova, Izmir, Turkey;3. Department of Histology, Ege University, 35100 Bornova, Izmir, Turkey;1. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China;2. Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UK;3. School of Plant Biology, University of Western Australia, Crawley, Perth 6009, Australia;4. Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan 666303, China;5. Chenshan Shanghai Botanical Garden, Shanghai, Songjiang, China;6. Research Center for Plant Conservation and Botanic Gardens, Indonesian Institute of Sciences (LIPI), Cibinong 16911, Indonesia;7. Fujian Agriculture and Forestry University, Shangxia Street 15, Changshan District, Fuzhou, Fujian, China;8. Southeast Asia Biodiversity Research Institute, Chinese Academy of Science (CAS-SEABRI), China;1. Key Laboratory for Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Heilongtan, Kunming 650201, Yunnan, China;2. Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China;3. Biological Sciences and Centre for Underutilized Crops, University of Southampton, Southampton, SO17 1BJ, United Kingdom;4. World Agroforestry Centre (ICRAF), P.O. Box 30677–00100, Nairobi, Kenya;5. University of Chinese Academy of Sciences, Beijing 100049, China;1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao;2. Information Technology College of Beijing Normal University Zhuhai Campus, Zhuhai City, China;3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China;4. State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu, Sichuan, China |
| |
| Abstract: | This study was aimed to authenticate and present phylogenetic relationship among 19 species of genus Chlorophytum using DNA barcoding. In all, 107 accessions were analyzed with eight plastid (matK, rbcL, trnH-psbA, rpoC1, ycf5, rpoB, atp and psbK-psbI) and six nuclear (ITS) markers. The matK and rbcL were found to be ideal markers for identification and discrimination of Chlorophytum species. Phylogenetic analysis based on matK and rbcL sequences resolved the species in two major clades. All markers, except matK and rbcL, showed ambiguous reads and paralogy in analysis. DGGE analysis showed the presence of pseudogenes and/or co-amplification in these markers, which caused poor sequence quality. Phylogeny and probable evolution of genus Chlorophytum was proposed on the basis of cytological, morphological and genetic information. |
| |
| Keywords: | Plant DNA barcoding Marker screening DGGE Pseudogenes SNP |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
