Effectiveness of ISSR markers for determination of the Aneura pinguis cryptic species and Aneura maxima |
| |
| Institution: | 1. Department of Genetics, Faculty of Biology, Adam Mickiewicz University, ul. Umultowska 89, 61-614 Poznań, Poland;2. Polish Academy of Sciences, Institute of Dendrology, Parkowa 5, 62-035 Kórnik, Poland;1. Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Boulevard Venustiano Carranza and José Cárdenas s/n, República Oriente, Saltillo 25280, Coahuila, Mexico;2. Clinical and Molecular Diagnosis Department, Universidad Autónoma de Coahuila, Boulevard Venustiano Carranza and José Cárdenas s/n, República Oriente, Saltillo 25280, Coahuila, Mexico;1. State Key Laboratory of Particle Detection and Electronics, Institute of High Energy Physics of Chinese Academy of Sciences, Beijing P. O. Box 100049, People’s Republic of China;2. Department of Physics, Nanjing University, Nanjing P. O. Box 210093, People’s Republic of China;1. Department of Physics, University of the Free State, P O Box 339, Bloemfontein, ZA9300, South Africa;2. Department of Physics, Shantou University, 243 Daxue Road, Shantou, 515063, Guangdong, China;1. Systematische Botanik und Mykologie, Department für Biologie I, Ludwig-Maximilians-Universität, Menzinger Str. 67, 80638 München, Germany;2. Mittlere Letten 11, 88634 Herdwangen-Schönach, Germany;3. Department of Botany, Swedish Museum of Natural History, Box 50007, SE-104 05 Stockholm, Sweden;4. Main Botanical Garden, Russian Academy of Sciences, Botanicheskaya 4, Moscow 127276, Russia;5. Courant Research Centre Geobiology, Georg-August-Universität Göttingen, Goldschmidtstraße 3, 37077 Göttingen, Germany;1. IICT-RMIT Research Centre, CSIR-Indian Institute of Chemical Technology, Hyderabad, India;2. Medicinal Chemistry & Pharmacology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, India;1. Department of Biology, College of Education for Women, Tikrit University, Tikrit, Iraq;2. The General Directorate for Education of Diyala, Diyala, Iraq;3. Biotecnology and Environmental Center, University of Fallujah, Fallujah, Iraq |
| |
| Abstract: | Aneura pinguis and Aneura maxima belong to the simple thalloid liverworts. Previous isozyme studies revealed that A. pinguis is a complex of cryptic species difficult to distinguished based on morphology. In the present study four cryptic species of the A. pinguis complex and A. maxima were examined by means of ISSR method to assess genetic variation and to develop species-specific markers. Eight ISSR primers used generated 460 bands, of which 453 were polymorphic. The highest values of resolving power 28.4 and marker index 18.1 were noted for primer 835 (AG)8-YC, while polymorphism information content for primer 842 (GA)7-AYG. The total gene diversity (HT) based on polymorphic loci was 0.284 for A. pinguis and 0.06 for A. maxima. ISSR markers supported existence of cryptic species in A. pinguis and showed genetic isolation between them. Species-specific bands were found for all studied cryptic species of A. pinguis and A. maxima, thus ISSRs can be used for their identification. A. maxima clearly differ from all the A. pinguis cryptic species in each amplified ISSR primer. The AMOVA conducted for the A. pinguis complex showed that most of genetic variation (ΦPT 0.586) was present among species. |
| |
| Keywords: | Thaloid liverworts Cryptic speciation Genetic variability Molecular markers Fingerprinting method |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
