Genetic diversity and structure among natural populations of Sindora glabra in Hainan Island,China as revealed by ISSR markers |
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| Institution: | 1. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China;2. Guangzhou Academy of Forestry and Landscape Gardening, Guangzhou, 510405, China;1. Molecular Ecology Laboratory, Department of Biological Oceanography, CICESE, Carretera Ensenada-Tijuana No. 3918, Ensenada, Baja California C.P. 22860, Mexico;2. NOAA Southwest Fisheries Science Center, 110 Shaffer Road, Santa Cruz, CA 95060, USA;3. School of Aquatic and Fishery Sciences, University of Washington, 1122 NE Boat St., Box 355020, Seattle, WA 98105, USA;4. Fisheries and Coastal Ecology Laboratory, Department of Marine Ecology, CICESE, Carretera Ensenada-Tijuana No. 3918, Ensenada, Baja California 22860, Mexico;1. Department of Breast Oncology, H. Lee Moffitt Cancer Center, Tampa, FL;2. Department of Diagnostic Imaging, H. Lee Moffitt Cancer Center, Tampa, FL;3. Biostatistic Department, Louisiana State University Health Sciences Center, School of Public Health, New Orleans, LA;1. Department of Chemistry, University of Agriculture, Faisalabad 38040, Pakistan;2. Institute of Food Science, Cornell University, NY 14852, USA;3. Department of Chemistry, Government College University, Lahore 54000, Pakistan;4. Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia;5. Department of Chemistry, University of Sargodha, Sargodha 40100, Pakistan;1. Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia;2. Indonesian Soil Research Institute, Jalan Ir. H. Juanda No. 98, Bogor 16123, Indonesia;3. Research Center for Bioresources and Biotechnology, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia;4. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia;5. Biopharmaca Research Center, Bogor Agricultural University, Jalan Taman Kencana No. 3, Bogor 16151, Indonesia;6. Graduate School of Information Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan |
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| Abstract: | Sindora glabra, one of the second-class national protective plants in China, has important ecological and commercial values. To understand the genetic diversity and structure of S. glabra, eleven natural populations from four areas of Hainan Island in China were analyzed by inter-simple sequence repeat (ISSR) markers. Thirteen primers were screened out and used to amplify 157 DNA samples. A total of 122 bands were obtained, among which 114 (93.4%) bands were polymorphic. Genetic parameters including average number of effective alleles (1.547), Nei's gene diversity (0.321), Shannon's information index (0.482), and gene differentiation coefficient (0.1944) revealed a high level of genetic diversity maintained in S. glabra populations. The variation within populations accounted for 85.6% of total variation based on analysis of molecular variance. Genetic distance analysis showed that 11 populations could be divided into three groups and populations from the same areas were classified as one group, suggesting that high genetic diversity of S. glabra was attributable to geographic isolation. Together, introduction of germplasm from distant natural distribution areas would be a sound strategy for germplasm resource conservation of S. glabra and selection of elite individuals from populations of far relationship for hybridization is of great importance for breeding improvement programs in future. |
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| Keywords: | ISSR Genetic diversity Conservation strategy |
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