Tirucallane Triterpenoids from the Stems of Brucea mollis |
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| Authors: | Hui Chen Shuang‐Gang Ma Zhen‐Feng Fang Jian Bai Shi‐Shan Yu Xiao‐Guang Chen Qi Hou Shao‐Peng Yuan Xia Chen |
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| Affiliation: | 1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P.?R. China (phone: +8610‐63165326, +8610‐60212124;2. fax: +8610‐63017757) |
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| Abstract: | Three new tirucallane triterpenoids, brumollisols A–C ( 1 – 3 , resp.), together with five known analogues, (23R,24S)‐23,24,25‐trihydroxytirucall‐7‐ene‐3,6‐dione ( 4 ), piscidinol A ( 5 ), 24‐epipiscidinol A ( 6 ), 21α‐methylmelianodiol ( 7 ), and 21β‐methylmelianodiol ( 8 ), were isolated from an EtOH extract of the stems of Brucea mollis. Their structures were elucidated by means of spectroscopic methods including 1D‐ and 2D‐NMR techniques and mass spectrometry. In the in vitro assays, compound 6 exhibited significant cytotoxic activity against A549 and BGC‐823 cancer cells with IC50 values of 1.16 and 3.01 μM , respectively. At a concentration of 10 μM , compounds 1 – 5, 7 , and 8 were found to inhibit NO production in mouse peritoneal macrophages with inhibitory ratios ranging from 39.8±7.7 to 68.2±4.5%. |
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| Keywords: | Tirucallanes Triterpenoids Brucea mollis Brumollisols A – C Inhibitors Cytotoxic activity |
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