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Alkaloids from Zephyranthes robusta Baker and Their Acetylcholinesterase‐ and Butyrylcholinesterase‐Inhibitory Activity |
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| Authors: | Andrea Kulhánková Lucie Cahlíková Zdeněk Novák Kateřina Macáková Jiří Kuneš Lubomír Opletal |
| Institution: | 1. ADINACO Research Group, Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles University, Heyrovského 1203, CZ‐500?05 Hradec Králové, (phone: +420?495?067?311;2. fax: +420?495?067?162);3. Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Charles University, Heyrovského 1203, CZ‐500?05 Hradec Králové |
| Abstract: | The bulbs of Zephyranthes robusta (Amaryllidaceae) have been extensively analyzed for their chemical constituents, resulting in the isolation of 13 alkaloids. The chemical structures of the isolated compounds were elucidated by mass‐spectrometric, and 1D‐ and 2D‐NMR spectroscopic experiments. The complete NMR assignments were achieved for hippeastidine. All isolated alkaloids were evaluated for their erythrocytic acetylcholinesterase and serum butyrylcholinesterase inhibitory activities using the Ellman's method. Significant acetylcholinesterase inhibition activity was exhibited by 8‐O‐demethylmaritidine (IC50(HuAChE) 28.0±0.9 μM ). |
| Keywords: | Zephyranthes robusta Alzheimer's disease Hippeastidine Acetylcholinesterase Butyrylcholinesterase Inhibitors |