Variability of n‐Alkanes and Nonacosan‐10‐ol in Natural Populations of Picea omorika |
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| Authors: | Biljana Nikolić Vele Tešević Iris Đorđević Marina Todosijević Milka Jadranin Srdjan Bojović Petar D. Marin |
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| Affiliation: | 1. Institute of Forestry, Kneza Vi?eslava 3, 11000 Belgrade, Serbia;2. Faculty of Chemistry, University of Belgrade, Studentski trg 16, P.O. Box 158, 11000 Belgrade, Serbia;3. Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobo?enja 18, 11000 Belgrade, Serbia;4. Institute for Chemistry, Technology, and Metallurgy, University of Belgrade, Njego?eva 12, 11000 Belgrade, Serbia;5. Institute for Biological Research ‘Sini?a Stankovi?‘, University of Belgrade, Boulevard Despota Stefana 142, 11060 Belgrade, Serbia;6. Institute of Botany and Botanical Garden ‘Jevremovac', Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia (phone: +38‐1113244498;7. fax: +38‐1113243603) |
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| Abstract: | This is the first report of population variability of the contents of n‐alkanes and nonacosan‐10‐ol in the needle epicuticular waxes of Serbian spruce (Picea omorika). The hexane extracts of needle samples originated from three natural populations in Serbia (Vranjak, Zmajeva?ki potok, and Mile?evka Canyon) were investigated by GC and GC/MS analyses. The amount of nonacosan‐10‐ol varied individually from 50.05 to 74.42% (65.74% in average), but the differences between the three investigated populations were not statistically confirmed. The results exhibited variability of the composition of n‐alkanes in the epicuticular waxes with their size ranging from C18 to C35. The most abundant n‐alkanes were C29, C31, and C27 (35.22, 13.77, and 12.28% in average, resp.). The carbon preference index of all the n‐alkanes (CPItotal) of the P. omorika populations (average of populations I–III) ranged from 3.3 to 11.5 (mean of 5.9), while the average chain length (ACL) ranged from 26.6 to 29.2. The principal component and cluster analyses of the contents of nine n‐alkanes showed the greatest difference for the population growing in the Mile?evka Canyon. The obtained results were compared with previous literature data given for other Picea species, and this comparison was briefly discussed. |
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| Keywords: | Picea omorika Nonacosan‐10‐ol Carbon preference index (CPI) Average chain length (ACL) Principal‐component analysis (PCA) Cluster analysis (CA) |
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