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Chromatography of human urinary erythropoietin and granulocyte colony-stimulating factor on insolubilized phytohaemagglutinin |
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| Authors: | Fritz Sieber |
| Institution: | 1. Friedrich Miescher-Institut, P.O. Box 273, CH-4002 Basel, Switzerland;2. Institut für Zellbiologie, Eidgenössiche Technische Hochschule, CH-8049 Zürich Switzerland |
| Abstract: | Human urinary erythropoietin was adsorbed to phytohaemagglutinin coupled to agarose or porous glass and quantitatively eluted by a saturated solution of MgCl2. This method provides a means of separating erythropoietin from several of its contaminants, presumably on the basis of its carbohydrate side chains. Erythropoietin which had been purified by chromatography on insolubilized phytohaemagglutinin was sufficiently free of toxicity to be assayable in tissue culture even when crude urine was used as a starting material. |
| Keywords: | epo U erythropoietin unit 1 epo U being defined as the amount of material stimulating the proliferation of erythroid progenitors in vitro [2] to the same extent as 5 mg of the Second International Reference Preparation of Erythropoietin [3] Tris tris(hydroxymethyl)-aminomethane |
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