Stereoselective Accumulation of Propranolol Enantiomers in K562 and K562/ADR Cells |
| |
| Authors: | Xiao‐Li Zheng Qin‐Qin Yu Yi Wang Su Zeng |
| |
| Institution: | 1. Lab of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, , Hangzhou, China;2. Research Institute, Puget Sound Blood Center, , Seattle, Washington |
| |
| Abstract: | The stereoselective uptake of propranolol enantiomers was investigated by using the K562 and K562 adriamycin‐resistant cell line (K562/ADR) as a model. An enantioselective RP‐HPLC method was applied to determine the accumulation of propranolol (PPL) stereoisomers in K562 and K562/ADR cells. The concentration, time and temperature dependent studies showed that the accumulation of S‐(?)‐PPL was higher than R‐(+)‐PPL in K562 cells and uptake of R‐(+)‐PPL was significantly higher than that of S‐(?)‐PPL in K562/ADR cells. The results indicate the enantioselective accumulation of propranolol enantiomers in K562 and K562 / ADR cells. Chirality 25:361–364, 2013. © 2013 Wiley Periodicals, Inc. |
| |
| Keywords: | K562 cells propranolol stereoselectivity accumulation |
|
|
