Identification of glutamine chain lengths of endogenous Folypoly-γ-glytamates in rat tissues |
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Authors: | Mary J Tyerman JE Watson B Shane DE Schutz ELR Stokstad |
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Institution: | Department of Nutritional Sciences, University of California, Berkeley, Calif. 94720 U.S.A. |
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Abstract: | A simplified procedure for the determination of the glutamate chain lengths of endogenous tissue folate is described.Natural pteroylpoly-γ-glutamates in tissue extracts, irrespective of their one-carbon moiety, were converted by a reductive cleavage procedure to a homologous series of p-aminobenzoylpoly-γ-glutamates, differing only in glutamate chain length. Desalting and concentration of the extracts were achieved by absorbing the derivatives on active charcoal followed by their elution with ethanol: ammonia. Aminobenzoylpolyglutamates were separated by DEAE- cellulose chromatography and quantitated by a colorimetric procedure for primary aromatic amines.The major endogenous folates in rat liver and kidney were pteroylpentaglutamate derivatives with smaller amounts of pteroyltetra- and hexaglutamate. |
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Keywords: | PteGlu pteroylglutamic acid folic acid tetrahydrofolic acid |
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