Extracellular matrix metabolism by chondrocytes III. Modulation of proteoglycan synthesis by extracellular levels of proteoglycan in cartilage cells in culture |
| |
Authors: | S. Handley D.A. Lowther |
| |
Affiliation: | Department of Biochemistry, Monash University, Clayton, Victoria 3168 Australia |
| |
Abstract: | Proteoglycan biosynthesis by cultured chondrocytes was shown to be depressed by extracellular concentrations of proteoglycan and partially degraded proteoglycan. This reduction in proteoglycan synthesis was reversible on removal of the added proteoglycan. , an exogenous acceptor of glycosaminoglycan synthesis, was used and it was shown that proteoglycan was inhibiting glycosaminoglycan synthesis. Proteoglycan had no effect on the overall protein synthesis by the cultured cells. It was concluded that the exogenous proteoglycan was inhibiting proteoglycan synthesis at the level of initiation or elongation of the glycosaminoglycan chains. |
| |
Keywords: | TES 2{2-hydroxy[1,1-bis(hydroxymethyl)ethyl]amino}ethanesulphonic acid BES 2[bis(2-hydroxyethyl)amino]ethanesulphonic acid HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulpnic acid |
本文献已被 ScienceDirect 等数据库收录! |