Involvement of xylem sap zeatin-O-glucoside in cucumber shoot greening |
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| Affiliation: | 1. Advanced Virtual and Intelligent Computing (AVIC) Center, Department of Mathematics and Computer Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand;2. Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand;3. Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand;1. Institute of Machinery Exploitation, Ergonomics and Production Processes, University of Agriculture in Krakow, ul. Balicka 116B, 31-149 Krakow, Poland;2. Department of Agrotechnology and Agricultural Ecology, University of Agriculture in Krakow, Krakow, Poland;3. Department of Agronomy, West Pomeranian University of Technology, Szczecin, Poland;1. Centro de Informática (CIn), Universidade Federal de Pernambuco (UFPE), Av. Jornalista Aníbal Fernandes, s/n - Cidade Universitária (Campus Recife) 50.740-560, Recife, PE, Brazil;2. Motorola Mobility LLC, 222 West Merchandise Mart Plaza, Chicago, Illinois 60654, USA |
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| Abstract: | A fraction of xylem sap collected from squash (Cucurbita maxima) root and separated with gel filtration showed strong promoting activity in a greening bioassay using etiolated cucumber cotyledons. This activity decreased with waterlogging of the root. The promoting factor was purified with sequential gel-permeation, and reverse-phase and normal-phase column chromatographies. Zeatin-O-glucoside (ZOG) was identified as the promoting factor by monitoring liquid chromatography tandem mass spectrometry. Although the glycosyl conjugate of zeatin was thought to be inactive, ZOG had a promoting activity that was 100 times higher than those of zeatin and zeatin riboside between concentrations of 10–9 to 10–4 M in the greening bioassay. These results suggest that in some developmental stages, the conjugation of cytokinins with a sugar moiety, such as glucose, might be a key factor in the control of shoot greening by roots. |
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