Relation between lipoic acid and cell redox status in wheat grown in excess copper |
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| Institution: | 1. College of Life Sciences, Nanjing Normal University, Nanjing 210097, China;2. Institute of Food and Crops, Jiangsu Academy of Agricultural Science, Nanjing 210014, China;3. Nanjing Sport Institute, Nanjing 210014, China;1. Department of Animal Scinces, Faculty of Agricultur, Bu-Ali Sina University, Hamedan, Iran;2. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran;3. Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;4. Department of Animal Scinces, Faculty of Agricultur and Natural Resources, University of Tehran, Karaj, Iran;1. Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, Yunnan 666303, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;3. Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, PR China;4. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, and College of Forestry, Guangxi University, Nanning, Guangxi 530004, PR China;1. Department of Biology, College of Haql, University of Tabuk, Tabuk, 71491, Saudi Arabia;2. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia;3. Department of Biology, Faculty of Science, University of Tabuk, Tabuk, 71491, Saudi Arabia;1. Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58108, USA;2. Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58108, USA;3. Department of Computer Science, North Dakota State University, Fargo, ND 58108, USA;1. College of Veterinary Medicine, Northeast Agricultural University, Harbin, China;2. Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, China;3. Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China |
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| Abstract: | Cell redox status and lipoic acid contents were analysed in wheat (Triticum durum Desf. cv. Creso) plants treated with 150 μM Cu to elucidate the role of the antioxidant lipoic acid against oxidative stress. In comparison with shoots, roots suffered a higher oxidative stress showing a decrease in NADPH contents and an oxidation of glutathione and ascorbate. Shoots did not evidence a clear oxidative damage since Cu was translocated in small amounts. Lipoic acid as reduced (DHLA) or oxidised (LA) form was present in both leaves and roots of wheat. Analysis of the cell sap showed that this antioxidant was present also as free form. The analyses showed that stroma contained significant amounts of free LA and that, after acidic hydrolysis, higher amounts of LA and DHLA were released. However, lipoic acid was undetectable in both thylakoids and microsomal membranes. Cu treatment did not determine changes in the contents of total LA and DHLA in roots, they being likely involved in Cu chelation. In contrast, in leaves after 48 h of treatment the metal induced an increase in DHLA, which could in part explain the reduction in the oxidised glutathione levels. In leaves free lipoic acid was more prone to be oxidised compared to the bound form, and the reduced form disappeared in both leaves and roots after Cu treatment. |
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