Effect of copper on peroxidase activity and lignin content in Raphanus sativus |
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| Institution: | 1. Department of Biology, Western Kentucky University, 1906 College Heights Blvd., Bowling Green, KY, 42101, USA;2. Plant Genetic Engineering and Molecular Biology Lab, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, 636 011, Tamil Nadu, India;1. School of Life Sciences, Hainan University, Haikou, 570228, China;2. State Key Laboratory of South China Sea Marine Resource Utilisation, Hainan University, Haikou, 570228, China;3. Hainan Academy of Ocean and Fisheries Sciences, Haikou, 571126, China;4. One Health Institute, Hainan University, Haikou, Hainan, 570228, China;5. School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China;1. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China;2. Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, Nanjing, 210095, China;3. Center for Multi-Omics Research, Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475004, China;4. Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021, China;1. Department of Botany, Government Degree College, Ramban, Jammu, 182144, India;2. Department of Botany, University of Jammu, Jammu and Kashmir, India;3. Centre for Applied Biology in Environment Sciences, Kurukshetra University, Kurukshetra, 136119, India;4. State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou, 311300, China;5. Independent Researcher, House No.282, Lane no. 3, Friends Colony, Opposite DAV College, Jalandhar, 144008, Punjab, India;6. Independent Researcher, House No. 472, Ward No. 8, Dhariwal, Gurdaspur, 143519, Punjab, India;7. Department of Botany, Dyal Singh College, Karnal, Haryana, 132001, India;8. Punjab Remote Sensing Centre, Ludhiana, India |
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| Abstract: | Copper (Cu2+) significantly inhibits the growth of radish (Raphanus sativus) seedlings at the concentration of 1 μM. As far as the relationship between the growth of radish roots and peroxidase (POD) activity is concerned, the reduction of radish roots is correlated with the induction of cationic and anionic PODs. The data show that the increase of cationic PODs (pI 8.6 and pI 9.3) and anionic PODs (pI 5.1 and pI 3.5) activities was correlated with the rise in lignin content in Cu-treated tissues. In our investigation, among the radish root PODs, the cationic pI 8.6 POD isozyme displayed a high affinity (Km of 57.9 μM) for syringaldazine and the similar value of catalytic efficiency jointly with the anionic pI 5.1 POD, 0.14 and 0.12 μM–1 s–1, respectively. The results suggest that the increase of cationic POD (pI 8.6) induced by Cu treatment can be a good candidate for lignification in radish roots. |
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