Isolation of wound inducible genes from Castanea sativa stems and expression analysis in the bark tissue |
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| Institution: | 1. State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China;2. Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA;3. Center for Cancer Molecular Diagnosis, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, China;4. State Key Laboratory of Protein and Plant Gene Research and Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, Peking University, Beijing 100871, China;5. Advanced Analytics Institute and Centre for Health Technologies, University of Technology Sydney, 81 Broadway, Sydney, NSW 2007, Australia;6. National Engineering Laboratory for Industrial Enzymes and Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;7. Infection and Immunity Program, Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Faculty of Medicine, Monash University, Melbourne, VIC 3800, Australia;8. Monash Centre for Data Science, Faculty of Information Technology, Monash University, Melbourne, VIC 3800, Australia;1. University of Stuttgart, Institute of Interfacial Process Engineering and Plasma Technology, Stuttgart, Germany;2. Fraunhofer-Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany;1. Departments of Pathology, Fukuoka University Hospital and School of Medicine, Fukuoka, Japan;2. Departments of Thoracic Surgery, Fukuoka University Hospital and School of Medicine, Fukuoka, Japan;3. Pathology and Cytology Center of the PCL Fukuoka, Fukuoka, Japan;4. Department of Pathology, Tokyo Women’s Medical University Yachiyo Medical Center, Yachiyo, Japan;5. Department of Pathology, Osaka Prefectural Hospital Organization, Osaka Prefectural Medical Center for Respiratory and Allergic Disease, Habikino, Japan;6. Department of Pathology, Hyogo College of Medicine, Hyogo, Japan;7. Department of Diagnostic Pathology, Graduate School of Medicine, Chiba University, Chiba, Japan;1. Univ Lyon, INSA Lyon, CNRS, Université Lyon 1, CPE Lyon, UMR 5246, ICBMS, Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires, Laboratoire de Chimie Organique et Bioorganique Bât. J. Verne, 20 avenue A. Einstein, F-69621 Villeurbanne, France;2. Univ Lyon, Université Lyon 1, CNRS, INSA Lyon, CPE Lyon, UMR 5246, ICBMS, Institut de Chimie et de Biochimie Moléculaires et Supramoléculaires, CASYEN Bât Curien (CPE) 43, Bd du 11 Novembre 1918, 69622 Villeurbanne cedex, France |
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| Abstract: | In vitro shoot cultures of chestnut (Castanea sativa Mill.) were used to identify wound-responsive genes. cDNA fragments of genes induced 3 and 24 h after wounding were isolated from stem tissue by the differential mRNA display method. Corresponding partial and full-length clones were isolated from a cDNA library of wounded stems. Putative wound-responsive signalling genes (serine–threonine protein kinase, two calmodulin genes), a novel wound-responsive putative chaperon gene (Csp13.9), and a new family of proline-rich proteins were identified. Northern analysis of bark tissue from 14-month-old seedlings revealed strong induction of these genes upon wounding in a temporal manner. Therefore we conclude that these early wound-inducible genes are involved in the wound response of bark tissue. |
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