Taxol transport in Taxus baccata cell suspension cultures |
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| Affiliation: | 1. Department of Radiation Oncology, Tungs'' Taichung Metro Harbor Hospital, Taichung, Taiwan;2. Division of Radiotherapy, Cheng Hsin General Hospital, Taipei, Taiwan;3. School of Medicine, National Yang-Ming University, Taipei, Taiwan;4. Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan;5. Department of Hemato-Oncology, Taichung Hospital, Ministry of Health and Welfare, Taichung, Taiwan;6. Cardiovascular Center and Department of Anesthesiology, Taichung Veteran General Hospital, Taichung, Taiwan;7. Department of Radiation Oncology, Taipei Medical University and Hospital, Taipei, Taiwan;8. Department of Radiation Oncology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;1. Department of Life Sciences, Presidency University, 86/1 College Street, Kolkata, West Bengal, India;3. MMHS, South 24 Pgs, West Bengal, India;3. Department of Biotechnology, Lovely Faculty of Technology and Sciences, Lovely Professional University, Phagwara, Punjab, India |
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| Abstract: | Taxol transport in Taxus baccata L. cell suspension cultures was studied using 〚14C〛-taxol as a tracer. The time course of uptake showed a saturable absorption that reached a maximum within 20 min. The uptake depended on its exogenous concentration and its accumulation was highly stimulated in the presence of 10–15 μM exogenous taxol. The absorbed molecule was found to localise both in the cell walls (20 %) and in the cell protoplasts (80 %), suggesting an accumulation within the vacuoles. Taxol uptake was strongly inhibited by Na-orthovanadate and verapamil, while Ca2+ was found to be one of the factors required for the active absorption of the molecule, since in the absence of this cation, the uptake was reduced by about 40 % and occurred mainly through a non-energy dependent mechanism. Taxol release into the culture medium was demonstrated not to depend on cell lysis, occurred through a mechanism that reached its maximum after 10–15 min and was strongly enhanced by treatment with Na-orthovanadate and verapamil, although the effect was found to be transient. |
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