PSCA和Oct-4蛋白在眼睑基底细胞癌组织中的表达及其意义

目的 探讨PSCA和Oct-4蛋白在眼睑基底细胞癌组织中的表达及其意义.方法 收集武汉市中心医院和武汉大学人民医院病理科2002～2009年手术切除及活检的眼睑基底细胞癌（basal cell carcinoma,BCC）标本共20例,另取癌周围组织5例作对照.采用免疫组织化学方法观察各组细胞内PSCA和Oct-4蛋白的表达.利用图像分析系统测定PSCA和Oct-4蛋白在以上各组中表达的平均光密度和平均阳性面积率.结果 （1）PSCA的表达在眼睑基底细胞癌中,PSCA呈高表达,而癌周围组织中PSCA呈低表达.经q 检验,眼睑基底细胞癌组织与癌旁组织之间PSCA的平均光密度及阳性面积率的差异有显著性意义（P〈0.05）.（2）Oct-4的表达眼睑基底细胞癌组织中Oct-4蛋白呈高表达;癌旁组织中Oct-4蛋白呈低表达.图像分析结果显示：眼睑基底细胞癌组织与癌旁组织之间Oct-4蛋白的平均光密度及阳性面积率的差异有显著性意义（P〈0.05）.结论 在眼睑基底细胞癌组织中PSCA和Oct-4蛋白的高表达可能是＂癌干细胞＂,PSCA和Oct-4蛋白也许参与了眼睑基底细胞癌的发生和发展.

Expression and Significance of PSCA and Oct-4 protein in basal cell carcinoma

Objective To research the expression and significance of PSCA and Oct-4 protein in basal cell carcinoma. Methods Twenty samples of surgical excision and biopsy of eyelid basal cell carcinoma （BCC） were collected from the departments of pathology of Wuhan Central Hospital and Wuhan University Zhongnan Hospital in 2002-2009, and 5 normal tissues around the cancer acted as controls. Immunohistochemical staining was performed to detect the expression of PSCA and Oct-4 protein. The average optical density and the rate of positive area of expression of PSCA and Oct-4 protein were analyzed using Image Analysis System. Results （1） The expression of PSCA was high in eyelid basal cell carcinoma, but low in normal tissue. Image analysis result showed that the expression of PSCA in eyelid basal cell carcinoma was significantly higher than that in normal tissue （P〈0.05）. （2） The expression of Oct-4 protein was high in eyelid basal cell carcinoma, but low in normal tissue. Image analysis result showed that the expression of Oct-4 protein in eyelid basal cell carcinoma was significantly higher than that in normal tissue （P〈0.05）. Conclusion PSCA and Oct-4 protein of high expression may be ＂cancer stem cells＂ in eyelid basal cell Car cinoma, PSCA and Oct-4 protein may participate in the occurrence and development of eyelid basal cell carelnoma.