| Abstract: | Studies were undertaken to further elucidate the mechanism(s) by which bradykinin-dependent phosphoinositide metabolism takes place in neuroblastoma X glioma hybrid NG108-15 cells (1984) J. Biol. Chem. 259, 10201-10207] using 3H]inositol-labelled cells. Bradykinin produced net increases in the level of 3H]inositol phosphates, especially of 3H]inositol trisphosphate which is formed transiently and most rapidly. The results indicate that bradykinin activates a phosphodiesterase to break down phosphatidylinositol 4,5-bisphosphate, generating two recently recognized intracellular messengers, 1,2-diacylglycerol and inositol trisphosphate. |
