Aerobic,inactive forms ofAzotobacter vinelandii hydrogenase: Activation kinetics and insensitivity to C2H2 inhibition |
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| Affiliation: | 1. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, PR China;2. School of Material Science and Engineering, Wuhan University of Technology, Wuhan 430070, PR China;3. National Research Tomsk Polytechnic University, Lenin Avenue 30, Tomsk 634050, Russian Federation;4. Global Campus Songdo, Ghent University, 119 Songdomunhwa-Ro, Yeonsu-Gu, Incheon, Republic of Korea;1. Greehey Children''s Cancer Research Institute, San Antonio, TX, 78229, USA |
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| Abstract: | Azotobacter vinelandii hydrogenase (EC class 1.12), either purified or membrane-associated, was obtained aerobically in an inactive state. The kinetics of activation by treatment with a reductant (H2 or dithionite) were determined. Three distinct phases of the activation were observed. Aerobically prepared, inactive hydrogenase was insensitive to acetylene inhibition, but could be rendered acetylene-sensitive by reduction with dithionite. These findings indicate that acetylene inhibition of hydrogenase requires catalytically active enzyme. |
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