Molecular cloning and nucleotide sequence of tuna growth hormone cDNA |
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| Institution: | 1. Research Institute for Food Science, Kyoto University, Uji Japan;2. Institute for Research and Development, Taiyo Fisheries Co., Ltd., Tokyo Japan;1. Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey;2. Department of Biology, Erzincan University, Uzumlu Vocational School, Erzincan, Turkey;3. Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Turkey;4. Department of Histology and Embryology, Faculty of Medicine, Ataturk University, Erzurum, Turkey;1. School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China;2. Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou, China;1. Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova (Padua), 35020 Legnaro (PD), Italy;2. Department of Veterinary Science, University of Parma, 43126 Parma, Italy;1. AgroBioSciences, Mohammed VI Polytechnic University, 43150 Ben-Guerir, Morocco;2. Laboratoire d’Ecologie, de Biologie et de Physiologie des Organismes Aquatiques, LR18ES41, Faculté des Sciences de Tunis, Université Tunis EL Manar, 2092 Tunis, Tunisia;3. Laboratory of Biotechnology and Biomonitoring of the Environment and Oasis Ecosystems, Faculty of Sciences of Gafsa, 2112 Gafsa, Tunisia;4. CHU ibn Eljazar, Unités Chirurgicales les Aglabides Kairouan, Service de Chirurgie Générale, Tunisie;5. Institut Supérieur des Sciences et Technologies de l’Environnement, Université de Carthage, Tunisia;6. Faculté des Sciences de Tunis (UR13/ES25), Université Tunis El - Manar, 2092, Tunis, Tunisia;1. Vin?a Institute of Nuclear Sciences, Laboratory for Physical Chemistry, University of Belgrade, Serbia;2. State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China;1. Department of Hematology and Oncology, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany;2. Institute of Clinical Radiology and Nuclear Medicine, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany;3. Department of Statistical Analysis, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany |
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| Abstract: | cDNA for mRNA of tuna growth hormone (GH) was cloned by screening a cDNA library constructed from tuna pituitary gland poly(A)+ RNA. The nucleotide sequence of cDNA (911 bases) revealed an open reading frame of 615 nucleotides, including a sequence (51 bases) for a possible secretory protein leader peptide. Noncoding regions were found in the nucleotide sequences up- (5′-terminal: 65 bases) and down- (3′-terminal: 231 bases) stream of the open reading frame. An amino-acid sequence deduced from the nucleotide sequence of the cDNA was identical with that determined in the purified tuna GH. Tuna GH was composed of 187 amino acids, and had a calculated molecular weight of 21275. Amino-acid sequencing showed that there was one possible N-glycosylation site at Asn (Asn-Cys-Thr). Tuna GH showed amino-acid sequence homologies with chum salmon (67%), yellow tail (90%) and with human (32%) growth hormones. |
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