Relationship between the induction of mitotic gene conversion and the formation of thymine glycols in yeast S. cerevisiae treated with hydrogen peroxide |
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| Institution: | 1. Biophysics Division, National Cancer Center Research Institute, Tsukiji 5-1-1, Chuo-ku, Tokyo Japan;2. Lawrence Berkeley Laboratory, Building 934, University of California, Berkeley, CA 94720 U.S.A.;3. Institute of Physics, College of General Education, University of Tokyo, Komaba 3-8-1, Meguroku, Tokyo Japan |
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| Abstract: | The effects of hydrogen peroxide on yeast Saccharomyces cerevisiae were assessed by measuring gene conversion at the trp 5 locus and the amount of thymine glycols in DNA using a monoclonal antibody specific to this base modification.Our results show that: (a) hydrogen peroxide-induced mitotic gene conversion in yeast strain D7M1 was dose-dependent in the low dose range where no toxicity was observed; (b) in the low dose range, the frequency of gene conversion depended on the temperature of the treatment, with more conversion at 25°C than at 15°C; (c) thymine glycols were induced in DNA in a dose-dependent manner following exposure of cells to up to 400 mM hydrogen peroxide; (d) there was little difference in the amount of thymine glycols formed in DNA when treatment occurred at either 25°C or 15°C. |
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