Drug test chamber: a titanium implant for administration of biochemical agents to a standardized bone callus in situ |
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| Affiliation: | 1. Department of Orthopaedics, University of Lund, S-221 85 Lund, Sweden;2. Department of Handicap Research and the Laboratory of Experimental Biology, University of Gothenburg, Sweden;3. Department of Anatomy, University of Gothenburg, Sweden;1. Assistant Professor, Terna Dental College Nerul, Mumbai, India; Former ITI Scholar, Department of Reconstructive Sciences University of Connecticut Health Center, Farmington, Conn;2. Former prosthodontics fellow, Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, Conn;3. Program Director, Department of Reconstructive Sciences, Post-Graduate Prosthodontics, University of Connecticut Health Center, Farmington, Conn;1. Dipartimento di Chimica e Farmacia, Università Degli Studi di Sassari, Via Vienna, 2, I-07100, Sassari, Italy;2. Dipartimento di Agraria, Università Degli Studi di Sassari, Viale Italia, 39A, I-07100, Sassari, Italy;3. Secció de Química Analítica, Facultat de Química, Universitat de Barcelona, Martí I Franquès, 1, E-08028, Barcelona, Spain;1. Mohammed Bin Rashid University of Medicine and Health Sciences, Hamdan Bin Mohammed College of Dental Medicine, Dubai Healthcare City, Dubai, United Arab Emirates;2. Honorary Associate Professor, Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand;3. Ras Al-Khaimah Dental Center, Ministry of Health and Prevention, United Arab Emirates;1. Department of Pharmacy, University of Salerno, 84084 Fisciano, SA, Italy;2. Department of Life and Environmental Sciences, University of Cagliari, 09124 Cagliari, Italy;3. Dipartimento di Scienze Farmaceutiche, University of Pisa, Pisa, Italy;4. NanoBioCel Group, School of Pharmacy, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, Spain;5. Department of Analytical Chemistry and Food Science and Technology, University of Castilla-La Mancha, Ronda de Calatrava 7, 13071 Ciudad Real, Spain;6. Regional Institute for Applied Scientific Research (IRICA), Area of Food Science, University of Castilla-La Mancha, Avenida Camilo Jose Cela, 10, 13071 Ciudad Real, Spain;7. Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Fuentenueva s/n, E-18071 Granada, Spain;8. Food Toxicology Unit, Department of Life and Environmental Science, University Campus of Monserrato, University of Cagliari, SS 554, Cagliari 09042, Italy;9. Biopharmaceutics and Pharmacokinetics Unit, Institute for Nanoscience and Nanotechnology, University of Barcelona, Barcelona, Spain;10. Department of Pharmacy and Pharmaceutical Technology and Parasitology, University of Valencia, Burjassot, 46100 Valencia, Spain |
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| Abstract: | A titanium implant in which a conduit is gradually filled with ingrowing bone (the Bone Harvest Chamber) has been modified to allow continuous local treatment of the conduit tissue with biochemical agents. Implants were inserted bilaterally in rabbit tibiae. The tissue content of the bone ingrowth conduits was studied with histology, 99mTc-MDP scintimetry and measurements of total calcium content. Bone was formed in the conduit by endochondral formation starting at both ends and continuing until fusion in the middle. After 2 weeks the bone had not yet met in the middle where fibrous tissue was seen. In eight animals 3H-proline was applied via one of the chambers, with the contralateral chamber as a saline-treated control. The collagen of the harvested tissue from the 3H-proline treated side had a 3H-hydroxyproline content 1000 times greater than had the control side. The ‘drug test chamber’ makes possible the study of local effects of drugs on healing of mature bone in vivo. |
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