Subcellular localization of Cd in the root cells of<Emphasis Type="Italic">Allium sativum</Emphasis> by electron energy loss spectroscopy |
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Authors: | Donghua Liu Ingrid Kottke |
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Institution: | Department of Biology, College of Chemistry and Life Sciences, Tianjin Normal University, Tianjin300074, People s Republic of China. donghua@mail.zlnet.com.cn |
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Abstract: | The ultrastructural investigation of the root cells ofAllium sativum L. exposed to three different concentrations of Cd (100 (AM, 1 μM and 10 mM) for 9 days was carried out. The results showed
that Cd induced several significant ultrastructural changes — high vacuolization in cytoplasm, deposition of electron-dense
material in vacuoles and nucleoli and increment of disintegrated organelles. Data from electron energy loss spectroscopy (EELS)
revealed that Cd was localized in the electron-dense precipitates in the root cells treated with 10 mM Cd. High amounts of
Cd were mainly accumulated in the vacuoles and nucleoli of cortical cells in differentiating and mature root tissues. The
mechanisms of detoxification and tolerance of Cd are briefly explained. |
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Keywords: | Allium sativum EELS Cd Gomori-Swift reaction |
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