| Abstract: | Membrane preparations from clover seedlings catalyzed the incorporation of monosaccharide residues from GDPMan, UDPGlc and UDPGal into glycolipids, lipid-oligosaccharides and polymers. The lipid-oligosaccharides were shown to be alpha-dihydropolyprenyl pyrophosphate derivatives. Incorporation of mannose residues into the lipid-oligosaccharides and the polymers was significantly stimulated by addition of UDPGlc, GDPGlc, UDPGal but not by UDPGlcNAc. Exogenic polyprenyl phosphates also stimulated the process; the formation of moraprenyl pyrophosphate oligosaccharides was demonstrated after addition of moraprenyl phosphate. The lipid-oligosaccharides were precursors of the polymers which were shown to be mainly glycoproteins. A solubilized preparation of mannosyl transferase from clover membranes was obtained and some properties of the enzyme were studied. |
