Plantlet regeneration from fascicular buds of seedling shoot apices of <Emphasis Type="Italic">Pinus roxburghii</Emphasis> Sarg |
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Authors: | R K Kalia S Arya S Kalia I D Arya |
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Institution: | (1) Tissue Culture Laboratory, Division of Genetics and Tree Propagation, Forest Research Institute, Dehradun, Uttaranchal, 248006, India;(2) Present address: Physical Mapping Laboratory, NRC on Plant Biotechnology, PUSA Campus, New Delhi, 110012, India |
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Abstract: | Shoot apices of Pinus roxburghii Sarg were cultured on Murashige and Skoog’s medium (MS) supplemented with cytokinins 6-benzyladenine (BA), kinetin and N-benzyl-9-(2-tetrahydropyranyl)
adenine (BPA)] alone and in combination with auxin, α-napthaleneacetic acid (NAA). Of the three cytokinins tested at varying
concentrations, medium supplemented with 10 μM BA was found optimal in respect of explant responsiveness (97.22 %) and average
number of buds induced per explant (7.42). The concentration of cytokinins in the induction medium had a profound effect on
rate of elongation of induced buds on MS basal medium containing 0.5 % activated charcoal. Further, shoots induced on lower
concentrations of BA increased up to 2.4 times in length in 4 weeks. Decapitation of the explant enhanced the rate of axillary
bud elongation. Proliferating shoot cultures were established by sub-culturing the axillary shoots on MS supplemented with
10 μM BA. Shoots 2–3 cm in length were suitable for culturing as more buds were induced on them compared to longer or shorter
shoots. Root primordia were induced on 70.83 % shoots when transferred to 1/2 MS medium supplemented with 5.0 μM NAA. Elongation
of root primordia (60 %) was achieved in liquid 1/2 MS basal medium. The plantlets were successfully transferred to soil after
hardening; the time period from initiation of shoot buds to transplantation being 20–22 weeks. |
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Keywords: | apical bud auxins axillary bud chir pine cytokinins micropropagation seedling |
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