Systems for the removal of a selection marker and their combination with a positive marker |
| |
Authors: | H Ebinuma K Sugita E Matsunaga S Endo K Yamada A Komamine |
| |
Institution: | Pulp and Paper Research Laboratory, Nippon Paper Industries Co, 5-21-1, Oji, Kita-ku, Tokyo 114-0002, Japan, The Research Institute of Evolutionary Biology, 2-4-28, Kamiyoga, Setagaya-ku, Tokyo, 158-0098, Japan,
|
| |
Abstract: | Many systems have been developed for the removal of a selection marker in order to generate marker-free transgenic plants. These systems consist of (1) a site-specific recombination system (Cre/lox) or a phage-attachment region (attP) to remove the selectable marker gene and (2) a transposable element system (Ac) or a co-transformation system to segregate the gene of interest from the selectable marker gene. Overall, the process is more time-consuming than conventional transformation methods because two rounds of transformation - two steps of regeneration or sexual crossings - are required to obtain the desired transgenic plants. Recently, removal systems combined with a positive marker, denoted as MAT vectors, have been developed to save time and effort by generating marker-free transgenic plants through a single-step transformation. We summarize here the transformation procedures using these systems and discuss their feasibility for practical use. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|